Influence of calcium ionophore on the fertilization of bovine oocytes and their further embryonic development

Intracytoplasmic spermatozoid injection (ICSI) is one of the commonest methods used in assisted reproductive technologies human medicine. However, this procedure has low efficacy for bovines, mainly because insufficient activation oocytes after microinjection. One most effective activating considered to be use phosphorus calcium, though optimal concentration activator and its effect on pre-implant development embyo are still open questions. An oocyte-cumulus complex clinically healthy cows, retrieved from ovaries during slaughter, matured over 22–24 h vitro conditions. Oocytes with visible polar body had been subjected intracytoplasmic (ICSI), were 15–30 min later activated environment different concentrations calcium ionophore 15–20 then transferred further cultivation a culture medium sodium pyruvate. The fertilization rate was identified second day at 2–4th stages cellular embryo, quality obtained embyos evaluated 8. Based statistical analysis data, we determined that artificial bovine using ionphore led statistically significant improvement conception ratio blastocytes injected. In study, confirmed addition 5, 10 50 µМ agent same occytes cattle. it noted zygotes up blastocyte stage (day 8), saw no differences embryos obtained. Therefore, effective, promotes increase parameters injected, facilitating production higher numbers suitable transplantation or cooling. Our previous conclusions valuable increasing purposes science production.